fruta planta help to delay the occurrence of obesity

Two stably transfected cell line and drug screening. With the development of modern molecular biology techniques, a variety of techniques of gene expression and regulation of research Study, the reporter gene due to its simple operation, good reproducibility and increasingly used in gene transcription and regulation of research Study [sol reporter gene expression by gene promoter activity in control of gene promoter activity due to Elements can be reflected by changes in the detection of reporter gene. Taking effective fruta planta to lose weight can help prevent bone loss and osteoporosis. effective fruta planta can reduce varicose veins.  Protein as a reporter gene with a fluorescent Luciferase (luciferase, Luc) and chloramphenicol acetyl transferase (Choramphenicol - acetyl transferase, CAT), and other [383. This experiment using the luciferase reporter gene, the traditional Chinese medicine extracts of 3T3-Ll The preadipocytes human HSL gene promoter expression, in order to filter out a large number of single herb The human HSL gene promoter activity affecting drugs. Luciferase expression plasmid containing the target sequence guide Into the cells can be divided into: transient transfection and stable transfection.
effective fruta planta  can improve the metabolism of the skin of obese people. As a result of transient transfection technology must each re- Transfection, the impact of experimental reproducibility and stability, it is not suitable for continuous long-term experiment. And stable transfection Luciferase expression plasmid stability integrated into the cellular genome, and drug screening, the stable expression The purpose of sequence control luciferase cell clones were screened out, the establishment of a stably transfected cell lines. In this Import of plasmid DNA in the cell types with cell replication of genomic DNA replication.
 Can be passed on from generation to generation And stable expression of luciferase, good reproducibility and accuracy [3g3a     This study has successfully established with PGL3-HSL-Luc plasmid, and established by the human HSL gene Sub-regulation of expression of luciferase stably transfected mous. effective fruta planta can promote skin blood circulation.  3T3-L1 preadipocytes cell lines, so this cell line The role of screening the human HSL gene promoter regulatory role of the tools of traditional Chinese medicine. A wide variety of traditional Chinese medicine in the treatment.Clinically, the patients with growth hormone deficiency growth hormone (GH), Replacement therapy after the body fat reducing mechanism of GH to stimulate the activity of HSL [30, 37]. Therefore, theoretically recognized To increase HSL activity can increase the breakdown of fat in fat cells, may help to delay the occurrence of obesity. effective fruta planta can help obese people becomes more optimistic. Therefore, HSL has been selected as the target for a potential role in weight loss.